• Immunohistochemistry analysis of DNA Ligase IV in paraffin-embedded human colon carcinoma using DNA Ligase IV Rabbit polyclonal antibody (STJ24407) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit polyclonal antibody (STJ24407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LIG4 antibody (1-210) (STJ24407)

SKU:
STJ24407

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIG4 (1-210) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIG4
Gene ID: 3981
Uniprot ID: DNLI4_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DNA Ligase IV (NP_002303.2).
Immunogen Sequence: MAASQTSQTVASHVPFADLC STLERIQKSKGRAEKIRHFR EFLDSWRKFHDALHKNHKDV TDSFYPAMRLILPQLERERM AYGIKETMLAKLYIELLNLP RDGKDALKLLNYRTPTGTHG DAGDFAMIAYFVLKPRCLQK GSLTIQQVNDLLDSIASNNS AKRKDLIKKSLLQLITQSSA LEQKWLIRMIIKDLKLGVSQ QTIFSVFHND
Tissue Specificity Testis, thymus, prostate and heart.
Function DNA ligase involved in DNA non-homologous end joining (NHEJ).required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4.the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. LIG4 regulates nuclear localization of XRCC4.
Protein Name Dna Ligase 4
Dna Ligase Iv
Polydeoxyribonucleotide Synthase Atp 4
Database Links Reactome: R-HSA-164843
Reactome: R-HSA-5693571
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Ligase 4 antibody
Anti-Dna Ligase Iv antibody
Anti-Polydeoxyribonucleotide Synthase Atp 4 antibody
Anti-LIG4 antibody

Information sourced from Uniprot.org

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