• Western blot analysis of extracts of various cell lines, using (STJ115468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using DNA Ligase III/LIG3 rabbit polyclonal antibody (STJ115468) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using DNA Ligase III/LIG3 rabbit polyclonal antibody (STJ115468) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using DNA Ligase III/LIG3 rabbit polyclonal antibody (STJ115468) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using DNA Ligase III/LIG3 rabbit polyclonal antibody (STJ115468) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using DNA Ligase III/LIG3 rabbit polyclonal antibody (STJ115468) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DNA Ligase III/LIG3 rabbit polyclonal antibody (STJ115468) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-LIG3 antibody (254-460) (STJ115468)

SKU:
STJ115468

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIG3 (254-460) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIG3
Gene ID: 3980
Uniprot ID: DNLI3_HUMAN
Immunogen Region: 254-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 254-460 of human DNA Ligase III/LIG3 (NP_039269.2).
Immunogen Sequence: CDPRHKDCLLREFRKLCAMV ADNPSYNTKTQIIQDFLRKG SAGDGFHGDVYLTVKLLLPG VIKTVYNLNDKQIVKLFSRI FNCNPDDMARDLEQGDVSET IRVFFEQSKSFPPAAKSLLT IQEVDEFLLRLSKLTKEDEQ QQALQDIASRCTANDLKCII RLIKHDLKMNSGAKHVLDAL DPNAYEAFKASRNLQDVVER VLHNAQE
Tissue Specificity Testis, thymus, prostate and heart.
Function Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair.
Protein Name Dna Ligase 3
Dna Ligase Iii
Polydeoxyribonucleotide Synthase Atp 3
Database Links Reactome: R-HSA-110381
Reactome: R-HSA-5649702
Reactome: R-HSA-5685939
Reactome: R-HSA-5696397
Reactome: R-HSA-6782210
Cellular Localisation Isoform 1: Mitochondrion
Contains An N-Terminal Mitochondrial Transit Peptide
Isoform 2: Mitochondrion
Isoform 3: Nucleus
Lacks The N-Terminal Mitochondrial Transit Peptide
Isoform 4: Nucleus
Alternative Antibody Names Anti-Dna Ligase 3 antibody
Anti-Dna Ligase Iii antibody
Anti-Polydeoxyribonucleotide Synthase Atp 3 antibody
Anti-LIG3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance