| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LEPREL1 (400-700) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
P3H2 |
| Gene ID: |
55214 |
| Uniprot ID: |
P3H2_HUMAN |
| Immunogen Region: |
400-700 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human LEPREL1 (NP_060662.2). |
| Immunogen Sequence: |
IRYGGRQDENRVPSGVNVEG AEVHGFSMGKKLSPKIDRDL REGGPLLYENITFVYNSEQL NGTQRVLLDNVLSEEQCREL HSVASGIMLVGDGYRGKTSP HTPNEKFEGATVLKALKSGY EGRVPLKSARLFYDISEKAR RIVESYFMLNSTLYFSYTHM VCRTALSGQQDRRNDLSHPI HADNCLLDPEANECWKEPPA YTFRDYSALLYMNDDFEGGE FIFTEMDAKTVTASIKPKC |
| Tissue Specificity | Expression localized to the epithelia of bile ducts and to the sacroplasm of heart muscle and skeletal muscle. In the pancreas, localized to a subpopulation of Langerhans islet cells and in the salivary gland, expressed in acinar cells (at protein level). Expressed in adult heart, placenta, lung, liver, skeletal muscle and kidney. Detected in fetal heart, spleen, lung, liver skeletal muscle and kidney. |
| Function | Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens. Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule. Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that lack 4-hydroxyproline in the third position. |
| Protein Name | Prolyl 3-Hydroxylase 2Leprecan-Like Protein 1Myxoid Liposarcoma-Associated Protein 4 |
| Database Links | Reactome: R-HSA-1650814 |
| Cellular Localisation | Endoplasmic ReticulumSarcoplasmic ReticulumGolgi Apparatus |
| Alternative Antibody Names | Anti-Prolyl 3-Hydroxylase 2 antibodyAnti-Leprecan-Like Protein 1 antibodyAnti-Myxoid Liposarcoma-Associated Protein 4 antibodyAnti-P3H2 antibodyAnti-LEPREL1 antibodyAnti-MLAT4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89094/357553/STJ11100022_1__83889.1713121675.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")