• Immunofluorescence analysis of paraffin-embedded human spleen using Lipocalin-2/NGAL Rabbit polyclonal antibody (STJ24381) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Lipocalin-2/NGAL in paraffin-embedded human esophagus tissue using Lipocalin-2/NGAL Rabbit polyclonal antibody (STJ24381) at a dilution of 1:900 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lipocalin-2/NGAL in paraffin-embedded human lung tissue using Lipocalin-2/NGAL Rabbit polyclonal antibody (STJ24381) at a dilution of 1:900 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lipocalin-2/NGAL in paraffin-embedded human spleen tissue using Lipocalin-2/NGAL Rabbit polyclonal antibody (STJ24381) at a dilution of 1:900 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Lipocalin-2/NGAL Rabbit polyclonal antibody (STJ24381) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LCN2 antibody (21-198) (STJ24381)

SKU:
STJ24381

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LCN2 (21-198) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCN2
Gene ID: 3934
Uniprot ID: NGAL_HUMAN
Immunogen Region: 21-198
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-198 of human Lipocalin-2/NGAL (NP_005555.2).
Immunogen Sequence: QDSTSDLIPAPPLSKVPLQQ NFQDNQFQGKWYVVGLAGNA ILREDKDPQKMYATIYELKE DKSYNVTSVLFRKKKCDYWI RTFVPGCQPGEFTLGNIKSY PGLTSYLVRVVSTNYNQHAM VFFKKVSQNREYFKITLYGR TKELTSELKENFIRFSKSLG LPENHIVFPVPIDQCIDG
Tissue Specificity Detected in neutrophils (at protein level). Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Expressed in the medullary tubules of the kidney. Not found in the small intestine or peripheral blood leukocytes.
Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis.
Protein Name Neutrophil Gelatinase-Associated Lipocalin
Ngal
25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9
Lipocalin-2
Oncogene 24p3
Siderocalin
P25
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Reactome: R-HSA-6799990
Reactome: R-HSA-917937
Cellular Localisation Secreted
Cytoplasmic Granule Lumen
Cytoplasmic Vesicle Lumen
Upon Binding To The Slc22a17 (24p3r) Receptor
It Is Internalized
Releases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles
Alternative Antibody Names Anti-Neutrophil Gelatinase-Associated Lipocalin antibody
Anti-Ngal antibody
Anti-25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9 antibody
Anti-Lipocalin-2 antibody
Anti-Oncogene 24p3 antibody
Anti-Siderocalin antibody
Anti-P25 antibody
Anti-LCN2 antibody
Anti-HNL antibody
Anti-NGAL antibody

Information sourced from Uniprot.org

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