Anti-LCAT antibody (6-56 aa) (STJ195174)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human LCAT at the amino acid range 6-56
General Information
| Short Description | Rabbit polyclonal anti-Phosphatidylcholine-sterol acyltransferase (6-56 aa) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LCAT |
| Gene ID | 3931 |
| Uniprot ID | LCAT_HUMAN |
| Immunogen | Synthesized peptide derived from the human LCAT at the amino acid range 6-56 |
| Immunogen Region | 6-56 aa |
| Specificity | This antibody detects endogenous levels of LCAT at Human/Mouse/Rat |
Additional Info
| Post Translational Modifications | O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures. |
| Function | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters. |
| Protein Name | Phosphatidylcholine-Sterol Acyltransferase1-Alkyl-2-Acetylglycerophosphocholine EsteraseLecithin-Cholesterol AcyltransferasePhospholipid-Cholesterol AcyltransferasePlatelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase |
| Database Links | Reactome: R-HSA-8964058 |
| Cellular Localisation | SecretedSecreted Into Blood PlasmaProduced In Astrocytes And Secreted Into Cerebral Spinal Fluid (Csf) |
| Alternative Antibody Names | Anti-Phosphatidylcholine-Sterol Acyltransferase antibodyAnti-1-Alkyl-2-Acetylglycerophosphocholine Esterase antibodyAnti-Lecithin-Cholesterol Acyltransferase antibodyAnti-Phospholipid-Cholesterol Acyltransferase antibodyAnti-Platelet-Activating Factor Acetylhydrolase antibodyAnti-Paf Acetylhydrolase antibodyAnti-LCAT antibody |
Information sourced from Uniprot.org