• Western blot analysis of lysates from mouse brain, using LCAT Rabbit polyclonal antibody (STJ113335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LCAT antibody (25-440) (STJ113335)

SKU:
STJ113335

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LCAT (25-440) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCAT
Gene ID: 3931
Uniprot ID: LCAT_HUMAN
Immunogen Region: 25-440
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-440 of human LCAT (NP_000220.1).
Immunogen Sequence: FWLLNVLFPPHTTPKAELSN HTRPVILVPGCLGNQLEAKL DKPDVVNWMCYRKTEDFFTI WLDLNMFLPLGVDCWIDNTR VVYNRSSGLVSNAPGVQIRV PGFGKTYSVEYLDSSKLAGY LHTLVQNLVNNGYVRDETVR AAPYDWRLEPGQQEEYYRKL AGLVEEMHAAYGKPVFLIGH SLGCLHLLYFLLRQPQAWKD RFIDGFISLGAPWGGSIKPM LVLASGDNQGIPIMSSIKL
Tissue Specificity Detected in blood plasma. Detected in cerebral spinal fluid (at protein level). Detected in liver. Expressed mainly in brain, liver and testes.
Post Translational Modifications O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures.
Function Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters.
Protein Name Phosphatidylcholine-Sterol Acyltransferase
1-Alkyl-2-Acetylglycerophosphocholine Esterase
Lecithin-Cholesterol Acyltransferase
Phospholipid-Cholesterol Acyltransferase
Platelet-Activating Factor Acetylhydrolase
Paf Acetylhydrolase
Database Links Reactome: R-HSA-8964058
Cellular Localisation Secreted
Secreted Into Blood Plasma
Produced In Astrocytes And Secreted Into Cerebral Spinal Fluid (Csf)
Alternative Antibody Names Anti-Phosphatidylcholine-Sterol Acyltransferase antibody
Anti-1-Alkyl-2-Acetylglycerophosphocholine Esterase antibody
Anti-Lecithin-Cholesterol Acyltransferase antibody
Anti-Phospholipid-Cholesterol Acyltransferase antibody
Anti-Platelet-Activating Factor Acetylhydrolase antibody
Anti-Paf Acetylhydrolase antibody
Anti-LCAT antibody

Information sourced from Uniprot.org

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