• Immunofluorescence analysis of HepG2 cells using LBP Rabbit polyclonal antibody (STJ119599) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using LBP antibody (STJ119599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using LBP antibody (STJ119599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LBP antibody (250-350) (STJ119599)

SKU:
STJ119599

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LBP (250-350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LBP
Gene ID: 3929
Uniprot ID: LBP_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human LBP (NP_004130.2).
Immunogen Sequence: EIFHRNHRSPVTLLAAVMSL PEEHNKMVYFAISDYVFNTA SLVYHEEGYLNFSITDDMIP PDSNIRLTTKSFRPFVPRLA RLYPNMNLELQGSVPSAPLL N
Tissue Specificity Detected in blood serum (at protein level).
Function Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Protein Name Lipopolysaccharide-Binding Protein
Lbp
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-166020
Reactome: R-HSA-5686938
Reactome: R-HSA-6785807
Cellular Localisation Secreted
Cytoplasmic Granule Membrane
Membrane-Associated In Polymorphonuclear Leukocytes (Pmn) Granules
Alternative Antibody Names Anti-Lipopolysaccharide-Binding Protein antibody
Anti-Lbp antibody
Anti-LBP antibody

Information sourced from Uniprot.org

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