• Immunoprecipitation analysis of 300 Mu g extracts of A-549 cells using 3 Mu g LASP1 antibody (STJ24376). Western blot was performed from the immunoprecipitate using LASP1 antibody (STJ24376) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using LASP1 Rabbit polyclonal antibody (STJ24376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using LASP1 Rabbit polyclonal antibody (STJ24376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using LASP1 Rabbit polyclonal antibody (STJ24376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using LASP1 Rabbit polyclonal antibody (STJ24376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LASP1 antibody (130-205) (STJ24376)

SKU:
STJ24376

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LASP1 (130-205) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LASP1
Gene ID: 3927
Uniprot ID: LASP1_HUMAN
Immunogen Region: 130-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-205 of human LASP1 (NP_006139.1).
Immunogen Sequence: RMGPSGGEGMEPERRDSQDG SSYRRPLEQQQPHHIPTSAP VYQQPQQQPVAQSYGGYKEP AAPVSIQRSAPGGGGK
Function Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
Protein Name Lim And Sh3 Domain Protein 1
Lasp-1
Metastatic Lymph Node Gene 50 Protein
Mln 50
Cellular Localisation Cytoplasm
Cell Cortex
Cytoskeleton
Associated With The F-Actin Rich Cortical Cytoskeleton
Alternative Antibody Names Anti-Lim And Sh3 Domain Protein 1 antibody
Anti-Lasp-1 antibody
Anti-Metastatic Lymph Node Gene 50 Protein antibody
Anti-Mln 50 antibody
Anti-LASP1 antibody
Anti-MLN50 antibody

Information sourced from Uniprot.org

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