| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LAPTM4B (218-317) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LAPTM4B |
| Gene ID: |
55353 |
| Uniprot ID: |
LAP4B_HUMAN |
| Immunogen Region: |
218-317 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 218-317 of human LAPTM4B (NP_060877.3). |
| Immunogen Sequence: |
NSIQEYIRQLPPNFPYRDDV MSVNPTCLVLIILLFISIIL TFKGYLISCVWNCYRYINGR NSSDVLVYVTSNDTTVLLPP YDDATVNGAAKEPPPPYVSA |
| Post Translational Modifications | Undergoes proteolytic cleavage following delivery to the lysosomes. Ubiquitinated by NEDD4. |
| Function | Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways. |
| Protein Name | Lysosomal-Associated Transmembrane Protein 4bLysosome-Associated Transmembrane Protein 4-Beta |
| Cellular Localisation | Endomembrane SystemMulti-Pass Membrane ProteinLate Endosome MembraneCell MembraneCell ProjectionLysosome MembraneEndosome MembraneEndosomeMultivesicular Body MembraneMultivesicular Body Lumen |
| Alternative Antibody Names | Anti-Lysosomal-Associated Transmembrane Protein 4b antibodyAnti-Lysosome-Associated Transmembrane Protein 4-Beta antibodyAnti-LAPTM4B antibodyAnti-PSEC0001 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
