• Western blot analysis of lysates from wild type (WT) and LAMTOR1 knockout (KO) HeLa cells, using [KO Validated] LAMTOR1 Rabbit polyclonal antibody (STJ11100859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LAMTOR1 antibody (1-161) (STJ11100859)

SKU:
STJ11100859

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LAMTOR1 (1-161) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LAMTOR1
Gene ID: 55004
Uniprot ID: LTOR1_HUMAN
Immunogen Region: 1-161
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1).
Immunogen Sequence: MGCCYSSENEDSDQDREERK LLLDPSSPPTKALNGAEPNY HSLPSARTDEQALLSSILAK TASNIIDVSAADSQGMEQHE YMDRARQYSTRLAVLSSSLT HWKKLPPLPSLTSQPHQVLA SEPIPFSDLQQVSRIAAYAY SALSQIRVDAKEELVVQFGI P
Post Translational Modifications N-terminal myristoylation and palmitoylation mediates its recruitment to lysosome membranes, thereby promoting localization of the Ragulator complex to lysosomes. N-myristoylation by NMT1 is required for palmitoylation at Cys-3 and Cys-4. May be palmitoylated by ZDHHC3. Ubiquitinated at Lys-60, Lys-103 and Lys-104 by UBE3A, promoting its degradation by the proteasome. Ubiquitination at Lys-20 impairs the association with the lysosomal V-ATPase complex. Deubiquitination at Lys-20 by USP32 promotes the association with the lysosomal V-ATPase complex and subsequent activation of the mTORC1 complex.
Function Key component of the Ragulator complex, a multiprotein complex involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to the lysosomal membrane. LAMTOR1 wraps around the other subunits of the Ragulator complex to hold them in place and interacts with the Rag GTPases, thereby playing a key role in the recruitment of the mTORC1 complex to lysosomes. Also involved in the control of embryonic stem cells differentiation via non-canonical RagC/RRAGC and RagD/RRAGD activation: together with FLCN, it is necessary to recruit and activate RagC/RRAGC and RagD/RRAGD at the lysosomes, and to induce exit of embryonic stem cells from pluripotency via non-canonical, mTOR-independent TFE3 inactivation. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.
Protein Name Ragulator Complex Protein Lamtor1
Late Endosomal/Lysosomal Adaptor And Mapk And Mtor Activator 1
Lipid Raft Adaptor Protein P18
Protein Associated With Drms And Endosomes
P27kip1-Releasing Factor From Rhoa
P27rf-Rho
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-6798695
Reactome: R-HSA-8943724
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9639288
Cellular Localisation Lysosome Membrane
Lipid-Anchor
Cytoplasmic Side
Late Endosome Membrane
Recruited To Lysosome And Endosome Membranes Through N-Terminal Myristoylation And Palmitoylation
Alternative Antibody Names Anti-Ragulator Complex Protein Lamtor1 antibody
Anti-Late Endosomal/Lysosomal Adaptor And Mapk And Mtor Activator 1 antibody
Anti-Lipid Raft Adaptor Protein P18 antibody
Anti-Protein Associated With Drms And Endosomes antibody
Anti-P27kip1-Releasing Factor From Rhoa antibody
Anti-P27rf-Rho antibody
Anti-LAMTOR1 antibody
Anti-C11orf59 antibody
Anti-PDRO antibody
Anti-PP7157 antibody

Information sourced from Uniprot.org

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