Western blot analysis of lysates from wild type (WT) and LAMTOR1 knockout (KO) HeLa cells, using [KO Validated] LAMTOR1 Rabbit polyclonal antibody (STJ11100859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LAMTOR1 antibody (1-161) (STJ11100859)

Name: Anti-LAMTOR1 antibody (1-161) (STJ11100859)
SKU: STJ11100859
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

ClonalityPolyclonal

HostRabbit

ConjugationUnconjugated

IsotypeIgG

STJ11100859

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General Information

Short Description	Rabbit polyclonal LAMTOR1 (1-161) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications	WB/IF/ICC/ELISA
Host	Rabbit
Reactivity	Human/Mouse/Rat
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Clonality	Polyclonal
Isotype	IgG
Conjugation	Unconjugated
Concentration	Lot specific
Purification	Affinity purification
Dilution Range	WB:1:500-1:1000 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation	PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage Instruction	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene Symbol	LAMTOR1
Gene ID	55004
Uniprot ID	LTOR1_HUMAN
Immunogen Region	1-161
Immunogen Sequence	MGCCYSSENEDSDQDREERK LLLDPSSPPTKALNGAEPNY HSLPSARTDEQALLSSILAK TASNIIDVSAADSQGMEQHE YMDRARQYSTRLAVLSSSLT HWKKLPPLPSLTSQPHQVLA SEPIPFSDLQQVSRIAAYAY SALSQIRVDAKEELVVQFGI P
Specificity	Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1).

Additional Info

Post Translational Modifications	N-terminal myristoylation and palmitoylation mediates its recruitment to lysosome membranes, thereby promoting localization of the Ragulator complex to lysosomes. N-myristoylation by NMT1 is required for palmitoylation at Cys-3 and Cys-4. May be palmitoylated by ZDHHC3. Ubiquitinated at Lys-60, Lys-103 and Lys-104 by UBE3A, promoting its degradation by the proteasome. Ubiquitination at Lys-20 impairs the association with the lysosomal V-ATPase complex. Deubiquitination at Lys-20 by USP32 promotes the association with the lysosomal V-ATPase complex and subsequent activation of the mTORC1 complex.
Function	Key component of the Ragulator complex, a multiprotein complex involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to the lysosomal membrane. LAMTOR1 wraps around the other subunits of the Ragulator complex to hold them in place and interacts with the Rag GTPases, thereby playing a key role in the recruitment of the mTORC1 complex to lysosomes. Also involved in the control of embryonic stem cells differentiation via non-canonical RagC/RRAGC and RagD/RRAGD activation: together with FLCN, it is necessary to recruit and activate RagC/RRAGC and RagD/RRAGD at the lysosomes, and to induce exit of embryonic stem cells from pluripotency via non-canonical, mTOR-independent TFE3 inactivation. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.
Protein Name	Ragulator Complex Protein Lamtor1 Late Endosomal/Lysosomal Adaptor And Mapk And Mtor Activator 1 Lipid Raft Adaptor Protein P18 Protein Associated With Drms And Endosomes P27kip1-Releasing Factor From Rhoa P27rf-Rho
Database Links	Reactome: R-HSA-1632852 Reactome: R-HSA-165159 Reactome: R-HSA-166208 Reactome: R-HSA-380972 Reactome: R-HSA-5628897 Reactome: R-HSA-6798695 Reactome: R-HSA-8943724 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013406 Reactome: R-HSA-9013407 Reactome: R-HSA-9013408 Reactome: R-HSA-9013409 Reactome: R-HSA-9013423 Reactome: R-HSA-9639288
Cellular Localisation	Lysosome Membrane Lipid-Anchor Cytoplasmic Side Late Endosome Membrane Recruited To Lysosome And Endosome Membranes Through N-Terminal Myristoylation And Palmitoylation
Alternative Antibody Names	Anti-Ragulator Complex Protein Lamtor1 antibody Anti-Late Endosomal/Lysosomal Adaptor And Mapk And Mtor Activator 1 antibody Anti-Lipid Raft Adaptor Protein P18 antibody Anti-Protein Associated With Drms And Endosomes antibody Anti-P27kip1-Releasing Factor From Rhoa antibody Anti-P27rf-Rho antibody Anti-LAMTOR1 antibody Anti-C11orf59 antibody Anti-PDRO antibody Anti-PP7157 antibody

Information sourced from Uniprot.org

Citations

View product citations for antibody STJ11100859 on CiteAb