Anti-LAMP2 antibody (121-170 aa) (STJ97664)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human LAMP2 at the amino acid range 121-170
General Information
| Short Description | Rabbit polyclonal anti-Lysosome-associated membrane glycoprotein 2 (121-170 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LAMP2 |
| Gene ID | 3920 |
| Uniprot ID | LAMP2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human LAMP2 at the amino acid range 121-170 |
| Immunogen Region | 121-170 aa |
| Specificity | CD107b Polyclonal Antibody detects endogenous levels of CD107b protein. |
Additional Info
| Post Translational Modifications | O- and N-glycosylated.some of the 16 N-linked glycans are polylactosaminoglycans. |
| Function | Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH, NLRP3 and MLLT11, and targeting them for lysosomal degradation. In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2. Plays a role in lysosomal protein degradation in response to starvation. Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation.antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits. Is not required for efficient MHC class II-mediated presentation of endogenous antigens. Isoform LAMP-2C: Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. |
| Protein Name | Lysosome-Associated Membrane Glycoprotein 2Lamp-2Lysosome-Associated Membrane Protein 2Cd107 Antigen-Like Family Member BLgp-96Cd Antigen Cd107b |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695Reactome: R-HSA-9613829 |
| Cellular Localisation | Lysosome MembraneSingle-Pass Type I Membrane ProteinEndosome MembraneCell MembraneCytoplasmic VesicleAutophagosome MembraneThis Protein Shuttles Between LysosomesEndosomesAnd The Plasma Membrane |
| Alternative Antibody Names | Anti-Lysosome-Associated Membrane Glycoprotein 2 antibodyAnti-Lamp-2 antibodyAnti-Lysosome-Associated Membrane Protein 2 antibodyAnti-Cd107 Antigen-Like Family Member B antibodyAnti-Lgp-96 antibodyAnti-Cd Antigen Cd107b antibodyAnti-LAMP2 antibody |
Information sourced from Uniprot.org