• Immunofluorescence analysis of U2OS cells using L2HGDH antibody (STJ117386) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Mouse heart, using L2HGDH Rabbit polyclonal antibody (STJ117386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-L2HGDH antibody (1-220) (STJ117386)

SKU:
STJ117386

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-L2HGDH (1-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: L2HGDH
Gene ID: 79944
Uniprot ID: L2HDH_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human L2HGDH (NP_079160.1).
Immunogen Sequence: MVPALRYLVGACGRARGLFA GGSPGACGFASGRPRPLCGG SRSASTSSFDIVIVGGGIVG LASARALILRHPSLSIGVLE KEKDLAVHQTGHNSGVIHSG IYYKPESLKAKLCVQGAALL YEYCQQKGISYKQCGKLIVA VEQEEIPRLQALYEKGLQNG VPGLRLIQQEDIKKKEPYCR GLMAIDCPHTGIVDYRQVAL SFAQDFQEAGGSVLTNFEVK
Tissue Specificity Widely expressed. Highly expressed in brain, testis and muscle. Expressed to a lower extent in lymphocytes, fibroblasts, keratinocytes, placenta, bladder, small intestine, liver and bone marrow.
Protein Name L-2-Hydroxyglutarate Dehydrogenase - Mitochondrial
Duranin
Database Links Reactome: R-HSA-880009
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-L-2-Hydroxyglutarate Dehydrogenase - Mitochondrial antibody
Anti-Duranin antibody
Anti-L2HGDH antibody
Anti-C14orf160 antibody

Information sourced from Uniprot.org

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