• Immunohistochemistry analysis of paraffin-embedded mouse heart using KYNU antibody (STJ28726) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using KYNU antibody (STJ28726) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : HAP1Exposure time: 45s.

Anti-KYNU antibody (1-307) (STJ28726)

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STJ28726

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KYNU (1-307) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KYNU
Gene ID: 8942
Uniprot ID: KYNU_HUMAN
Immunogen Region: 1-307
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human KYNU (NP_001028170.1).
Immunogen Sequence: MEPSSLELPADTVQRIAAEL KCHPTDERVALHLDEEDKLR HFRECFYIPKIQDLPPVDLS LVNKDENAIYFLGNSLGLQP KMVKTYLEEELDKWAKIAAY GHEVGKRPWITGDESIVGLM KDIVGANEKEIALMNALTVN LHLLMLSFFKPTPKRYKILL EAKAFPSDHYAIESQLQLHG LNIEESMRMIKPREGEETLR IEDILEVIEKEGDSIAVILF SGVHFYTGQHFNIPAITKA
Tissue Specificity Expressed in all tissues tested (heart, brain placenta, lung, liver, skeletal muscle, kidney and pancreas). Highest levels found in placenta, liver and lung. Expressed in all brain regions.
Function Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
Protein Name Kynureninase
L-Kynurenine Hydrolase
Database Links Reactome: R-HSA-71240
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Kynureninase antibody
Anti-L-Kynurenine Hydrolase antibody
Anti-KYNU antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance