• Western blot analysis of extracts of various cell lines, using Cytokeratin 7 (KRT7) (KRT7) rabbit monoclonal antibody (STJ11102055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human breast using Cytokeratin 7 (KRT7) rabbit monoclonal antibody (STJ11102055) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using Cytokeratin 7 (KRT7) rabbit monoclonal antibody (STJ11102055) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using Cytokeratin 7 (KRT7) rabbit monoclonal antibody (STJ11102055) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Human lung using KRT7 antibody (STJ11102055) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse lung using KRT7 antibody (STJ11102055) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat lung using KRT7 antibody (STJ11102055) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-KRT7 antibody (291-463) [S5MR] (STJ11102055)

SKU:
STJ11102055

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 7 (291-463) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT7
Gene ID: 3855
Uniprot ID: K2C7_HUMAN
Immunogen Region: 291-463
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 291-463 of human Cytokeratin 7 (KRT7) (P08729).
Immunogen Sequence: QAQAGKHGDDLRNTRNEISE MNRAIQRLQAEIDNIKNQRA KLEAAIAEAEERGELALKDA RAKQEELEAALQRGKQDMAR QLREYQELMSVKLALDIEIA TYRKLLEGEESRLAGDGVGA VNISVMNSTGGSSSGGGIGL TLGGTMGSNALSFSSSAGPG LLKAYSIRTASAS
Tissue Specificity Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Post Translational Modifications Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Protein Name Keratin - Type Ii Cytoskeletal 7
Cytokeratin-7
Ck-7
Keratin-7
K7
Sarcolectin
Type-Ii Keratin Kb7
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 7 antibody
Anti-Cytokeratin-7 antibody
Anti-Ck-7 antibody
Anti-Keratin-7 antibody
Anti-K7 antibody
Anti-Sarcolectin antibody
Anti-Type-Ii Keratin Kb7 antibody
Anti-KRT7 antibody
Anti-SCL antibody

Information sourced from Uniprot.org

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