• 1) Input: Hela Cell Lysate , 2) IP product: IP dilute 1:200
  • IHC staining of human lung cancer tissue, diluted at 1:200.
  • Immunofluorescence analysis of human-liver tissue. 1, CK7 monoclonal antibody (12D7) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 1) Hela, 2) Mouse Kidney, 3) Mouse Brain, diluted at 1:2000.
  • Immunofluorescence analysis of Hela cell. 1, FoxO1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-KRT7 antibody [12D7] (STJ96956)

SKU:
STJ96956

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IF/WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type II cytoskeletal 7 is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 12D7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IF 1:50-200
WB 1:1000-2000
IHC 1:200
IP 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT7
Gene ID: 3855
Uniprot ID: K2C7_HUMAN
Specificity: The antibody detects endogenous CK7 proteins.
Immunogen: Synthetic Peptide of CK7
Post Translational Modifications Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Protein Name Keratin - Type Ii Cytoskeletal 7
Cytokeratin-7
Ck-7
Keratin-7
K7
Sarcolectin
Type-Ii Keratin Kb7
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 7 antibody
Anti-Cytokeratin-7 antibody
Anti-Ck-7 antibody
Anti-Keratin-7 antibody
Anti-K7 antibody
Anti-Sarcolectin antibody
Anti-Type-Ii Keratin Kb7 antibody
Anti-KRT7 antibody
Anti-SCL antibody

Information sourced from Uniprot.org

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