• Immunoprecipitation analysis of 200 Mu g extracts of A-431 cells using 3 Mu g Cytokeratin 5 (Cytokeratin 5 (KRT5) ) antibody (STJ24360). Western blot was performed from the immunoprecipitate using Cytokeratin 5 (Cytokeratin 5 (KRT5) ) antibody (STJ24360) at a dilution of 1:5000.
  • Immunofluorescence analysis of L929 cells using Cytokeratin 5 (Cytokeratin 5 (KRT5) ) Rabbit polyclonal antibody (STJ24360) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cytokeratin 5 (KRT5) in paraffin-embedded Human esophageal cancer using Cytokeratin 5 (Cytokeratin 5 (KRT5) ) Rabbit polyclonal antibody (STJ24360) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Cytokeratin 5 (KRT5) Rabbit polyclonal antibody (STJ24360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT5 antibody (1-100) (STJ24360)

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STJ24360

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KRT5 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:2000-1:6000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT5
Gene ID: 3852
Uniprot ID: K2C5_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 5 (KRT5) (NP_000415.2).
Immunogen Sequence: MSRQSSVSFRSGGSRSFSTA SAITPSVSRTSFTSVSRSGG GGGGGFGRVSLAGACGVGGY GSRSLYNLGGSKRISISTSG GSFRNRFGAGAGGGYGFGGG
Tissue Specificity Expressed in corneal epithelium (at protein level). Expressed in keratinocytes (at protein level).
Post Translational Modifications Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle. Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis. CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury. O-glycosylated.
Function Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress. Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes.
Protein Name Keratin - Type Ii Cytoskeletal 5
58 Kda Cytokeratin
Cytokeratin-5
Ck-5
Keratin-5
K5
Type-Ii Keratin Kb5
Database Links Reactome: R-HSA-446107
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 5 antibody
Anti-58 Kda Cytokeratin antibody
Anti-Cytokeratin-5 antibody
Anti-Ck-5 antibody
Anti-Keratin-5 antibody
Anti-K5 antibody
Anti-Type-Ii Keratin Kb5 antibody
Anti-KRT5 antibody

Information sourced from Uniprot.org

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