• Western blot analysis of extracts of H460 cells, using KRT3 Antibody (STJ112157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of A431 cells using KRT3 rabbit polyclonal antibody (STJ112157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-KRT3 antibody (230-390) (STJ112157)

SKU:
STJ112157

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KRT3 (230-390) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT3
Gene ID: 3850
Uniprot ID: K2C3_HUMAN
Immunogen Region: 230-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-390 of human KRT3 (NP_476429.2).
Immunogen Sequence: WNLLQQQGTSSISGTNNLEP LFENHINYLRSYLDNILGER GRLDSELKNMEDLVEDFKKK YEDEINKRTAAENEFVTLKK DVDSAYMNKVELQAKVDALI DEIDFLRTLYDAELSQMQSH ISDTSVVLSMDNNRSLDLDS IIAEVRAQYEDIAQRSKAEA E
Tissue Specificity Cornea specific.
Protein Name Keratin - Type Ii Cytoskeletal 3
65 Kda Cytokeratin
Cytokeratin-3
Ck-3
Keratin-3
K3
Type-Ii Keratin Kb3
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 3 antibody
Anti-65 Kda Cytokeratin antibody
Anti-Cytokeratin-3 antibody
Anti-Ck-3 antibody
Anti-Keratin-3 antibody
Anti-K3 antibody
Anti-Type-Ii Keratin Kb3 antibody
Anti-KRT3 antibody

Information sourced from Uniprot.org

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