• Immunofluorescence analysis of MCF7 cells using Cytokeratin 19 (KRT19) Rabbit polyclonal antibody (STJ24355) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cytokeratin 19 (KRT19) in paraffin-embedded human tonsil using Cytokeratin 19 (KRT19) Rabbit polyclonal antibody (STJ24355) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cytokeratin 19 (KRT19) in paraffin-embedded human placenta using Cytokeratin 19 (KRT19) Rabbit polyclonal antibody (STJ24355) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cytokeratin 19 (KRT19) in paraffin-embedded human colon using Cytokeratin 19 (KRT19) Rabbit polyclonal antibody (STJ24355) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Cytokeratin 19 (KRT19) Rabbit polyclonal antibody (STJ24355) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KRT19 antibody (210-400) (STJ24355)

SKU:
STJ24355

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KRT19 (210-400) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT19
Gene ID: 3880
Uniprot ID: K1C19_HUMAN
Immunogen Region: 210-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-400 of human Cytokeratin 19 (KRT19) (NP_002267.2).
Immunogen Sequence: ELAYLKKNHEEEISTLRGQV GGQVSVEVDSAPGTDLAKIL SDMRSQYEVMAEQNRKDAEA WFTSRTEELNREVAGHTEQL QMSRSEVTDLRRTLQGLEIE LQSQLSMKAALEDTLAETEA RFGAQLAHIQA
Tissue Specificity Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Function Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Protein Name Keratin - Type I Cytoskeletal 19
Cytokeratin-19
Ck-19
Keratin-19
K19
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 19 antibody
Anti-Cytokeratin-19 antibody
Anti-Ck-19 antibody
Anti-Keratin-19 antibody
Anti-K19 antibody
Anti-KRT19 antibody

Information sourced from Uniprot.org

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