• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma (A) , hepatocarcinoma (B) , stomach cancer (C) and colon cancer tissue (D) , showing cytoplasmic location with DAB staining using Cytokeratin 18 monoclonal antibody.
  • Western blot analysis using Cytokeratin 18 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , A549 (3) , Jurkat (4) , MCF-7 (5) , HepG2 (6) , A431 (7) , HEK293 (8) and K562 (9) cell lysate.

Anti-KRT18 antibody (391-483 aa) [4D11E4] (STJ97984)

SKU:
STJ97984

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type I cytoskeletal 18 (391-483 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 4D11E4
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT18
Gene ID: 3875
Uniprot ID: K1C18_HUMAN
Immunogen Region: 391-483 aa
Specificity: Cytokeratin 18 Monoclonal Antibody detects endogenous levels of Cytokeratin 18 protein.
Immunogen: Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
Post Translational Modifications Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Protein Name Keratin - Type I Cytoskeletal 18
Cell Proliferation-Inducing Gene 46 Protein
Cytokeratin-18
Ck-18
Keratin-18
K18
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Nucleus Matrix
Cytoplasm
Perinuclear Region
Nucleus
Nucleolus
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 18 antibody
Anti-Cell Proliferation-Inducing Gene 46 Protein antibody
Anti-Cytokeratin-18 antibody
Anti-Ck-18 antibody
Anti-Keratin-18 antibody
Anti-K18 antibody
Anti-KRT18 antibody
Anti-CYK18 antibody
Anti-PIG46 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance