Anti-KRT18 antibody (391-483 aa) [4D11E4] (STJ97984)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Keratin, type I cytoskeletal 18 (391-483 aa) for use in WB, IHC, IF and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4D11E4 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000IHC 1:200-1:1000ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KRT18 |
| Gene ID | 3875 |
| Uniprot ID | K1C18_HUMAN |
| Immunogen | Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli. |
| Immunogen Region | 391-483 aa |
| Specificity | Cytokeratin 18 Monoclonal Antibody detects endogenous levels of Cytokeratin 18 protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
| Function | Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
| Protein Name | Keratin - Type I Cytoskeletal 18Cell Proliferation-Inducing Gene 46 ProteinCytokeratin-18Ck-18Keratin-18K18 |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | Nucleus MatrixCytoplasmPerinuclear RegionNucleusNucleolus |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 18 antibodyAnti-Cell Proliferation-Inducing Gene 46 Protein antibodyAnti-Cytokeratin-18 antibodyAnti-Ck-18 antibodyAnti-Keratin-18 antibodyAnti-K18 antibodyAnti-KRT18 antibodyAnti-CYK18 antibodyAnti-PIG46 antibody |
Information sourced from Uniprot.org