• Western blot analysis of lysate from MCF7 cells, using KRT14 Antibody.
  • Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Western blot analysis of MCF7 cells using Cytokeratin 14 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-KRT14 antibody (421-470 aa) (STJ96538)

SKU:
STJ96538

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Keratin, type I cytoskeletal 14 (421-470 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT14
Gene ID: 3861
Uniprot ID: K1C14_HUMAN
Immunogen Region: 421-470 aa
Specificity: Cytokeratin 14 Polyclonal Antibody detects endogenous levels of Cytokeratin 14 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human KRT14 at the amino acid range 421-470
Post Translational Modifications A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Protein Name Keratin - Type I Cytoskeletal 14
Cytokeratin-14
Ck-14
Keratin-14
K14
Database Links Reactome: R-HSA-446107
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Expressed In Both As A Filamentous Pattern
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 14 antibody
Anti-Cytokeratin-14 antibody
Anti-Ck-14 antibody
Anti-Keratin-14 antibody
Anti-K14 antibody
Anti-KRT14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance