Anti-KRT14 antibody (400-472 aa) [PT1674] (STJ196754)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenSynthesized peptide derived from the human Cytokeratin 14 at the amino acid range 400-472
General Information
| Short Description | Mouse monoclonal anti-Keratin, type I cytoskeletal 14 (400-472 aa) for use in IHC, WB and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB/IF |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | PT1674 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 500-2000IHC-P 1:100-500IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KRT14 |
| Gene ID | 3861 |
| Uniprot ID | K1C14_HUMAN |
| Immunogen | Synthesized peptide derived from the human Cytokeratin 14 at the amino acid range 400-472 |
| Immunogen Region | 400-472 aa |
| Specificity | This antibody detects endogenous levels of human Cytokeratin 14. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section. The antib |
Additional Info
| Post Translational Modifications | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. |
| Function | The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. |
| Protein Name | Keratin - Type I Cytoskeletal 14Cytokeratin-14Ck-14Keratin-14K14 |
| Database Links | Reactome: R-HSA-446107Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | CytoplasmNucleusExpressed In Both As A Filamentous Pattern |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 14 antibodyAnti-Cytokeratin-14 antibodyAnti-Ck-14 antibodyAnti-Keratin-14 antibodyAnti-K14 antibodyAnti-KRT14 antibody |
Information sourced from Uniprot.org