Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit polyclonal antibody (STJ116946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT12 antibody (295-494) (STJ116946)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ116946
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General Information

Short DescriptionRabbit polyclonal KRT12 (295-494) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolKRT12
Gene ID3859
Uniprot IDK1C12_HUMAN
Immunogen Region295-494
Immunogen SequenceTRLLNDMRAQYETIAEQNRK DAEAWFIEKSGELRKEISTN TEQLQSSKSEVTDLRRAFQN LEIELQSQLAMKKSLEDSLA EAEGDYCAQLSQVQQLISNL EAQLLQVRADAERQNVDHQR LLNVKARLELEIETYRRLLD GEAQGDGLEESLFVTDSKSQ AQSTDSSKDPTKTRKIKTVV QEMVNGEVVSSQVQEIEELM
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human Cytokeratin 12 (Cytokeratin 12 (KRT12) ) (NP_000214.1).

Additional Info

Tissue Specificity Expressed in the corneal epithelium (at protein level).
Function Involved in corneal epithelium organization, integrity and corneal keratin expression.
Protein Name Keratin - Type I Cytoskeletal 12
Cytokeratin-12
Ck-12
Keratin-12
K12
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 12 antibody
Anti-Cytokeratin-12 antibody
Anti-Ck-12 antibody
Anti-Keratin-12 antibody
Anti-K12 antibody
Anti-KRT12 antibody

Information sourced from Uniprot.org

Citations

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