• Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 1 (KRT1) Rabbit monoclonal antibody (STJ11102547, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of extracts of A375 cells, using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Confocal imaging of paraffin-embedded Mouse skin using Cytokeratin 1 (KRT1) Rabbit monoclonal antibody (STJ11102547, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded rat skin using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens).
  • Confocal imaging of paraffin-embedded Human skin using Cytokeratin 1 (KRT1) Rabbit monoclonal antibody (STJ11102547, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse skin using Cytokeratin 1 (KRT1) (KRT1) Rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse skin using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Cytokeratin 1 (KRT1) (KRT1) Rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat skin using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat skin using Cytokeratin 1 (KRT1) (KRT1) Rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human skin using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of A375 cells, using Cytokeratin 1 (KRT1) (KRT1) Rabbit monoclonal antibody (STJ11102547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of mouse tail and ear using Cytokeratin 1 (KRT1) (KRT1) rabbit monoclonal antibody (STJ11102547) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) (KRT1) Rabbit monoclonal antibody (STJ11102547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT1 antibody (545-644) [S7MR] (STJ11102547)

SKU:
STJ11102547

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 1 (545-644) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT1
Gene ID: 3848
Uniprot ID: K2C1_HUMAN
Immunogen Region: 545-644
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Cytokeratin 1 (KRT1) (P04264).
Immunogen Sequence: GGGGRGSYGSGGSSYGSGGG SYGSGGGGGGHGSYGSGSSS GGYRGGSGGGGGGSSGGRGS GGGSSGGSIGGRGSSSGGVK SSGGSSSVKFVSTTYSGVTR
Tissue Specificity The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Post Translational Modifications Undergoes deimination of some arginine residues (citrullination).
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Protein Name Keratin - Type Ii Cytoskeletal 1
67 Kda Cytokeratin
Cytokeratin-1
Ck-1
Hair Alpha Protein
Keratin-1
K1
Type-Ii Keratin Kb1
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cell Membrane
Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 1 antibody
Anti-67 Kda Cytokeratin antibody
Anti-Cytokeratin-1 antibody
Anti-Ck-1 antibody
Anti-Hair Alpha Protein antibody
Anti-Keratin-1 antibody
Anti-K1 antibody
Anti-Type-Ii Keratin Kb1 antibody
Anti-KRT1 antibody
Anti-KRTA antibody

Information sourced from Uniprot.org

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