• Biotinylated STJ70491 (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-KPNA3/IPOA4, Biotinylated antibody (C-Term) {Biotin} (STJ73288)

SKU:
STJ73288-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC/ICC/IF/IP
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-KPNA3/IPOA4, Biotinylated (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: KPNA3
Gene ID: 3839
Uniprot ID: IMA4_HUMAN
Immunogen Region: C-Term
Accession Number: NP_002258.2
Immunogen Sequence: CDPTANLQTKEFNF.
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
Protein Name Importin Subunit Alpha-4
Importin Alpha Q2
Qip2
Karyopherin Subunit Alpha-3
Srp1-Gamma
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-168276
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Importin Subunit Alpha-4 antibody
Anti-Importin Alpha Q2 antibody
Anti-Qip2 antibody
Anti-Karyopherin Subunit Alpha-3 antibody
Anti-Srp1-Gamma antibody
Anti-KPNA3 antibody
Anti-QIP2 antibody

Information sourced from Uniprot.org

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