• Western blot analysis of extracts of various cell lines, using KPNA3 antibody (STJ110645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using KPNA3 Rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat kidney using KPNA3 rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of L929 cells using KPNA3 Rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA3 rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of H9C2 cells using KPNA3 Rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using KPNA3 rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreas using KPNA3 Rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat stomach using KPNA3 rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human esophageal using KPNA3 Rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal using KPNA3 rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using KPNA3 Rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using KPNA3 rabbit polyclonal antibody (STJ110645) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using KPNA3 antibody (STJ110645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-KPNA3 antibody (1-210) (STJ110645)

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STJ110645

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KPNA3 (1-210) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KPNA3
Gene ID: 3839
Uniprot ID: IMA4_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human KPNA3 (NP_002258.2).
Immunogen Sequence: MAENPSLENHRIKSFKNKGR DVETMRRHRNEVTVELRKNK RDEHLLKKRNVPQEESLEDS DVDADFKAQNVTLEAILQNA TSDNPVVQLSAVQAARKLLS SDRNPPIDDLIKSGILPILV KCLERDDNPSLQFEAAWALT NIASGTSAQTQAVVQSNAVP LFLRLLRSPHQNVCEQAVWA LGNIIGDGPQCRDYVISLGV VKPLLSFISP
Tissue Specificity Ubiquitous. Highest levels in heart and skeletal muscle.
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
Protein Name Importin Subunit Alpha-4
Importin Alpha Q2
Qip2
Karyopherin Subunit Alpha-3
Srp1-Gamma
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-168276
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Importin Subunit Alpha-4 antibody
Anti-Importin Alpha Q2 antibody
Anti-Qip2 antibody
Anti-Karyopherin Subunit Alpha-3 antibody
Anti-Srp1-Gamma antibody
Anti-KPNA3 antibody
Anti-QIP2 antibody

Information sourced from Uniprot.org

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