Anti-KPNA2 antibody [R04-3C7] (STJA0036627)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human KPNA2
STJA0036627
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General Information

Short DescriptionRabbit monoclonal anti-KPNA2 for use in WB and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/FC
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR04-3C7
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
FC 1:50-1:100
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolKPNA2
Gene ID3838
Uniprot IDIMA1_HUMAN
ImmunogenA synthesized peptide derived from human KPNA2

Additional Info

Tissue Specificity Expressed ubiquitously.
Post Translational Modifications Ubiquitinated. Deubiquitinated by USP22.leading to stabilization and increased activity.
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import.acts redundantly with KPNA1 and Transportin-1/TNPO1.
Protein Name Importin Subunit Alpha-1
Karyopherin Subunit Alpha-2
Rag Cohort Protein 1
Srp1-Alpha
Database Links Reactome: R-HSA-111932
Reactome: R-HSA-1169408
Reactome: R-HSA-168276
Reactome: R-HSA-442729
Reactome: R-HSA-5693548
Reactome: R-HSA-9018519
Reactome: R-HSA-9692916
Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Nucleus
Endoplasmic Reticulum Membrane
Golgi Apparatus Membrane
(Microbial Infection) Retained In Er/Golgi Membranes Upon Interaction With Sars-Cov Virus Orf6 Protein
Alternative Antibody Names Anti-Importin Subunit Alpha-1 antibody
Anti-Karyopherin Subunit Alpha-2 antibody
Anti-Rag Cohort Protein 1 antibody
Anti-Srp1-Alpha antibody
Anti-KPNA2 antibody
Anti-RCH1 antibody
Anti-SRP1 antibody

Information sourced from Uniprot.org

Citations

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