| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KPNA1 (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KPNA1 |
| Gene ID: |
3836 |
| Uniprot ID: |
IMA5_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KPNA1 (NP_002255.3). |
| Immunogen Sequence: |
MTTPGKENFRLKSYKNKSLN PDEMRRRREEEGLQLRKQKR EEQLFKRRNVATAEEETEEE VMSDGGFHEAQISNMEMAPG GVITSDMIEMIFSKSPEQQL SATQKFRKLLSKEPNPPIDE VISTPGVVARFVEFLKRKEN CTLQFESAWVLTNIASGNSL QTRIVIQAGAVPIFIELLSS EFEDVQEQAVWALGNIAGDS TMCRDYVLDCNILPPLLQLF SKQNRLTMTRNAVWALSNL |
| Tissue Specificity | Expressed ubiquitously. |
| Post Translational Modifications | Polyubiquitinated in the presence of RAG1 (in vitro). |
| Function | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. |
| Protein Name | Importin Subunit Alpha-5Karyopherin Subunit Alpha-1Nucleoprotein Interactor 1Npi-1Rag Cohort Protein 2Srp1-Beta Cleaved Into - Importin Subunit Alpha-5 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-140342Reactome: R-HSA-162592Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-180910Reactome: R-HSA-68616Reactome: R-HSA-909733Reactome: R-HSA-9636249 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Importin Subunit Alpha-5 antibodyAnti-Karyopherin Subunit Alpha-1 antibodyAnti-Nucleoprotein Interactor 1 antibodyAnti-Npi-1 antibodyAnti-Rag Cohort Protein 2 antibodyAnti-Srp1-Beta Cleaved Into - Importin Subunit Alpha-5 - N-Terminally Processed antibodyAnti-KPNA1 antibodyAnti-RCH2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")