• Western blot analysis of extracts of various cell lines, using KPNA1 antibody (STJ115582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-KPNA1 antibody (1-300) (STJ115582)

SKU:
STJ115582

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KPNA1 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KPNA1
Gene ID: 3836
Uniprot ID: IMA5_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KPNA1 (NP_002255.3).
Immunogen Sequence: MTTPGKENFRLKSYKNKSLN PDEMRRRREEEGLQLRKQKR EEQLFKRRNVATAEEETEEE VMSDGGFHEAQISNMEMAPG GVITSDMIEMIFSKSPEQQL SATQKFRKLLSKEPNPPIDE VISTPGVVARFVEFLKRKEN CTLQFESAWVLTNIASGNSL QTRIVIQAGAVPIFIELLSS EFEDVQEQAVWALGNIAGDS TMCRDYVLDCNILPPLLQLF SKQNRLTMTRNAVWALSNL
Tissue Specificity Expressed ubiquitously.
Post Translational Modifications Polyubiquitinated in the presence of RAG1 (in vitro).
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Protein Name Importin Subunit Alpha-5
Karyopherin Subunit Alpha-1
Nucleoprotein Interactor 1
Npi-1
Rag Cohort Protein 2
Srp1-Beta Cleaved Into - Importin Subunit Alpha-5 - N-Terminally Processed
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-140342
Reactome: R-HSA-162592
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-180910
Reactome: R-HSA-68616
Reactome: R-HSA-909733
Reactome: R-HSA-9636249
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Importin Subunit Alpha-5 antibody
Anti-Karyopherin Subunit Alpha-1 antibody
Anti-Nucleoprotein Interactor 1 antibody
Anti-Npi-1 antibody
Anti-Rag Cohort Protein 2 antibody
Anti-Srp1-Beta Cleaved Into - Importin Subunit Alpha-5 - N-Terminally Processed antibody
Anti-KPNA1 antibody
Anti-RCH2 antibody

Information sourced from Uniprot.org

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