Anti-KMT5A antibody (1-100) [S1MR] (STJ11102001)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal KMT5A/SETD8 (1-100) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S1MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KMT5A |
| Gene ID | 387893 |
| Uniprot ID | KMT5A_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MGEGGAAAALVAAAAAAAAA AAAVVAGQRRRRLGRRARCH GPGRAAGGKMSKPCAVEAAA AAVAATAPGPEMVERRGPGR PRTDGENVFTGQSKIYSYMS |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KMT5A/SETD8 (Q9NQR1). |
Additional Info
| Post Translational Modifications | Acetylated at Lys-162.does not affect methyltransferase activity. Deacetylated at Lys-162 possibly by SIRT2.does not change methyltransferase activity. Ubiquitinated and degraded by the DCX(DTL) complex. |
| Function | Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. |
| Protein Name | N-Lysine Methyltransferase Kmt5aH4-K20-Hmtase Kmt5aHistone-Lysine N-Methyltransferase Kmt5aLysine N-Methyltransferase 5aLysine-Specific Methylase 5aPr/Set Domain-Containing Protein 07Pr-Set7Pr/Set07Set Domain-Containing Protein 8 |
| Database Links | Reactome: R-HSA-2299718Reactome: R-HSA-3214841Reactome: R-HSA-6804760 |
| Cellular Localisation | NucleusChromosomeSpecifically Localizes To Mitotic ChromosomesColocalized With Sirt2 At Mitotic FociAssociates With Chromosomes During MitosisAssociation Is Increased In A H(2)O(2)-Induced Oxidative Stress-Dependent MannerAssociates With Silent Chromatin On Euchromatic ArmsNot Associated With Constitutive Heterochromatin |
| Alternative Antibody Names | Anti-N-Lysine Methyltransferase Kmt5a antibodyAnti-H4-K20-Hmtase Kmt5a antibodyAnti-Histone-Lysine N-Methyltransferase Kmt5a antibodyAnti-Lysine N-Methyltransferase 5a antibodyAnti-Lysine-Specific Methylase 5a antibodyAnti-Pr/Set Domain-Containing Protein 07 antibodyAnti-Pr-Set7 antibodyAnti-Pr/Set07 antibodyAnti-Set Domain-Containing Protein 8 antibodyAnti-KMT5A antibodyAnti-PRSET7 antibodyAnti-SET07 antibodyAnti-SET8 antibodyAnti-SETD8 antibody |
Information sourced from Uniprot.org