| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KMT2E (200-1858) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KMT2E |
| Gene ID: |
55904 |
| Uniprot ID: |
KMT2E_HUMAN |
| Immunogen Region: |
200-1858 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 200-1858 of human MLL5 (NP_061152.3). |
| Immunogen Sequence: |
VELYTAFQHTPTSITLTASR VSKVNDKRRKKSGEKEQHIS KCKKAFREGSRKSSRVKGSA PEIDPSSDGSNFGWETKIKA WMDRYEEANNNQYSEGVQRE AQRIALRLGNGNDKKEMNKS DLNTNNLLFKPPVESHIQKN KKILKSAKDLPPDALIIEYR GKFMLREQFEANGYFFKRPY PFVLFYSKFHGLEMCVDART FGNEARFIRRSCTPNAEVRH EIQDGTIHLYIYSIHSIPK |
| Tissue Specificity | Widely expressed in both adult and fetal tissues. Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum. Isoform NKp44L: Not detected on circulating cells from healthy individuals, but is expressed on a large panel of tumor and transformed cells. |
| Post Translational Modifications | Ubiquitinated. Deubiquitinated by USP7. O-glycosylated at Ser-435 and Thr-440 in the SET domain by OGT which probably prevents KMT2E proteasomal-mediated degradation. |
| Function | Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription. Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry. Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition. During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Isoform NKp44L: Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity. |
| Protein Name | Inactive Histone-Lysine N-Methyltransferase 2eInactive Lysine N-Methyltransferase 2eMyeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 5 |
| Cellular Localisation | ChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleus SpeckleAbsent From The NucleolusLocalizes To Chromosome During Interphase And To Centrosomes During MitosisDissociation From Mitotic Chromosome Is Likely Due To Histone H3 Phosphorylation On 'Thr-3' And 'Thr-6'Isoform 3: NucleusNucleoplasmLocalizes To Chromosome During Interphase And To Nucleus Speckle During MitosisIsoform Nkp44l: CytoplasmCell MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Inactive Histone-Lysine N-Methyltransferase 2e antibodyAnti-Inactive Lysine N-Methyltransferase 2e antibodyAnti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 5 antibodyAnti-KMT2E antibodyAnti-MLL5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
