| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-Klotho (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | IHC-4-6µg/mlELISA-antibody detection limit dilution 1:4000. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | KL |
| Gene ID: | 9365 |
| Uniprot ID: | KLOT_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_004786.2; NP_710150.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_004786.2; NP_710150.1). |
| Immunogen Sequence: | KRDDAKYMYYLKK |
| Post Translational Modifications | N-glycosylated. |
| Function | May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. |
| Protein Name | Klotho Cleaved Into - Klotho Peptide |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-190374Reactome: R-HSA-2219530Reactome: R-HSA-5654219Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654726Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinApical Cell MembraneIsoform 1 Shedding Leads To A Soluble PeptideIsoform 2: SecretedKlotho Peptide: Secreted |
| Alternative Antibody Names | Anti-Klotho Cleaved Into - Klotho Peptide antibodyAnti-KL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
