• Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g KLF4 Rabbit monoclonal antibody (STJ11101428). Western blot was performed from the immunoprecipitate using KLF4 Rabbit monoclonal antibody (STJ11101428) at a dilution of 1:1000.
  • Confocal imaging of U-2 OS cells using KLF4 Rabbit monoclonal antibody (STJ11101428, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of KLF4 in paraffin-embedded mouse kidney using KLF4 Rabbit monoclonal antibody (STJ11101428) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KLF4 in paraffin-embedded mouse brain using KLF4 Rabbit monoclonal antibody (STJ11101428) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KLF4 in paraffin-embedded human liver cancer using KLF4 Rabbit monoclonal antibody (STJ11101428) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KLF4 in paraffin-embedded human colon using KLF4 Rabbit monoclonal antibody (STJ11101428) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse lung, using KLF4 Rabbit monoclonal antibody (STJ11101428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using KLF4 Rabbit monoclonal antibody (STJ11101428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KLF4 antibody (300-400) [S8MR] (STJ11101428)

SKU:
STJ11101428

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-KLF4 (300-400) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF4
Gene ID: 9314
Uniprot ID: KLF4_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KLF4 (O43474).
Immunogen Sequence: PAAHDFPLGRQLPSRTTPTL GLEEVLSSRDCHPALPLPPG FHPHPGPNYPSFLPDQMQPQ VPPLHYQGQSRGFVARAGEP CVCWPHFGTHGMMLTPPSSP L
Post Translational Modifications Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis. Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6.deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis.
Function Transcription factor.can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Protein Name Krueppel-Like Factor 4
Epithelial Zinc Finger Protein Ezf
Gut-Enriched Krueppel-Like Factor
Database Links Reactome: R-HSA-381340
Reactome: R-HSA-422085
Reactome: R-HSA-452723
Reactome: R-HSA-9617828
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Krueppel-Like Factor 4 antibody
Anti-Epithelial Zinc Finger Protein Ezf antibody
Anti-Gut-Enriched Krueppel-Like Factor antibody
Anti-KLF4 antibody
Anti-EZF antibody
Anti-GKLF antibody

Information sourced from Uniprot.org

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