• Western blot analysis of extracts of various cell lines, using KLC3 antibody (STJ116553) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-KLC3 antibody (1-135) (STJ116553)

SKU:
STJ116553

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLC3 (1-135) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLC3
Gene ID: 147700
Uniprot ID: KLC3_HUMAN
Immunogen Region: 1-135
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human KLC3 (NP_803136.2).
Immunogen Sequence: MSVQVAAPGSAGLGPERLSP EELVRQTRQVVQGLEALRAE HHGLAGHLAEALAGQGPAAG LEMLEEKQQVVSHSLEAIEL GLGEAQVLLALSAHVGALEA EKQRLRSQARRLAQENVWLR EELEETQRRLRASEE
Function Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa. May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece.
Protein Name Kinesin Light Chain 3
Klc2-Like
Kinesin Light Chain 2
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-5625970
Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Mitochondrion
In Elongating Spermatid Tail Midpiece
Localized In Outer Dense Fibers (Odfs) And Associates With Mitochondria
Also Localizes To The Manchette In Elongating Spermatids
Alternative Antibody Names Anti-Kinesin Light Chain 3 antibody
Anti-Klc2-Like antibody
Anti-Kinesin Light Chain 2 antibody
Anti-KLC3 antibody
Anti-KLC2 antibody
Anti-KLC2L antibody

Information sourced from Uniprot.org

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