• Western blot analysis of various lysates using KLB Rabbit polyclonal antibody (STJ118077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KLB antibody (720-990) (STJ118077)

SKU:
STJ118077

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLB (720-990) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLB
Gene ID: 152831
Uniprot ID: KLOTB_HUMAN
Immunogen Region: 720-990
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 720-990 of human KLB (NP_783864.1).
Immunogen Sequence: ALAWRLYDRQFRPSQRGAVS LSLHADWAEPANPYADSHWR AAERFLQFEIAWFAEPLFKT GDYPAAMREYIASKHRRGLS SSALPRLTEAERRLLKGTVD FCALNHFTTRFVMHEQLAGS RYDSDRDIQFLQDITRLSSP TRLAVIPWGVRKLLRWVRRN YGDMDIYITASGIDDQALED DRLRKYYLGKYLQEVLKAYL IDKVRIKGYYAFKLAEEKSK PRFGFFTSDFKAKSSIQFY
Function Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
Protein Name Beta-Klotho
Bkl
Betaklotho
Klotho Beta-Like Protein
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1307965
Reactome: R-HSA-2219530
Reactome: R-HSA-5654228
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5654720
Reactome: R-HSA-5654733
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Cellular Localisation Cell Membrane
Single-Pass Type Iii Membrane Protein
Alternative Antibody Names Anti-Beta-Klotho antibody
Anti-Bkl antibody
Anti-Betaklotho antibody
Anti-Klotho Beta-Like Protein antibody
Anti-KLB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance