Anti-KL antibody (1-200) (STJ113929)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KL (1-200) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KL |
| Gene ID | 9365 |
| Uniprot ID | KLOT_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MPASAPPRRPRPPPPSLSLL LVLLGLGGRRLRAEPGDGAQ TWARFSRPPAPEAAGLFQGT FPDGFLWAVGSAAYQTEGGW QQHGKGASIWDTFTHHPLAP PGDSRNASLPLGAPSPLQPA TGDVASDSYNNVFRDTEALR ELGVTHYRFSISWARVLPNG SAGVPNREGLRYYRRLLERL RELGVQPVVTLYHWDLPQRL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KL (NP_004786.2). |
Additional Info
| Tissue Specificity | Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney. |
| Post Translational Modifications | N-glycosylated. |
| Function | May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. |
| Protein Name | Klotho Cleaved Into - Klotho Peptide |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-190374Reactome: R-HSA-2219530Reactome: R-HSA-5654219Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654726Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinApical Cell MembraneIsoform 1 Shedding Leads To A Soluble PeptideIsoform 2: SecretedKlotho Peptide: Secreted |
| Alternative Antibody Names | Anti-Klotho Cleaved Into - Klotho Peptide antibodyAnti-KL antibody |
Information sourced from Uniprot.org