• STJ73013 (1µg/ml) staining of Human Hippocampus lysate (A) + peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73013 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73013 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73013 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-KIT/CD117 antibody (Internal) (STJ73013)

SKU:
STJ73013-100

£303.50
Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IF/IHC/FC
Reactivity: Human/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-KIT/CD117 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-10µg/ml
IF-Strong expression of the protein seen in the cytoplasm/membranes of HEK293 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: KIT
Gene ID: 3815
Uniprot ID: KIT_HUMAN
Immunogen Region: Internal
Accession Number: NP_000213.1; NP_001087241.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_000213.1; NP_001087241.1).
Immunogen Sequence: KRDSFICSKQEDH
Post Translational Modifications Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Protein Name Mast/Stem Cell Growth Factor Receptor Kit
Scfr
Piebald Trait Protein
Pbt
Proto-Oncogene C-Kit
Tyrosine-Protein Kinase Kit
P145 C-Kit
V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog
Cd Antigen Cd117
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-1433557
Reactome: R-HSA-1433559
Reactome: R-HSA-2219530
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8866910
Reactome: R-HSA-9669914
Reactome: R-HSA-9669917
Reactome: R-HSA-9669921
Reactome: R-HSA-9669924
Reactome: R-HSA-9669926
Reactome: R-HSA-9669929
Reactome: R-HSA-9669933
Reactome: R-HSA-9669934
Reactome: R-HSA-9669935
Reactome: R-HSA-9669936
Reactome: R-HSA-9670439
Reactome: R-HSA-9680187
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Isoform 3: Cytoplasm
Detected In The Cytoplasm Of Spermatozoa
Especially In The Equatorial And Subacrosomal Region Of The Sperm Head
Alternative Antibody Names Anti-Mast/Stem Cell Growth Factor Receptor Kit antibody
Anti-Scfr antibody
Anti-Piebald Trait Protein antibody
Anti-Pbt antibody
Anti-Proto-Oncogene C-Kit antibody
Anti-Tyrosine-Protein Kinase Kit antibody
Anti-P145 C-Kit antibody
Anti-V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog antibody
Anti-Cd Antigen Cd117 antibody
Anti-KIT antibody
Anti-SCFR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance