• Western blot analysis of extracts of various cell lines, using KIFC3 antibody (STJ111344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using KIFC3 antibody (STJ111344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KIFC3 antibody (140-390) (STJ111344)

SKU:
STJ111344

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIFC3
Gene ID: 3801
Uniprot ID: KIFC3_HUMAN
Immunogen Region: 140-390
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 140-390 of human KIFC3 (NP_005541.3).
Immunogen Sequence: MVENERLRQEMRRCEAELQE LRTKPAGPCPGCEHSQESAQ LRDKLSQLQLEMAESKGMLS ELNLEVQQKTDRLAEVELRL KDCLAEKAQEEERLSRRLRD SHETIASLRAQSPPVKYVIK TVEVESSKTKQALSESQARN QHLQEQVAMQRQVLKEMEQQ LQSSHQLTARLRAQIAMYES ELERAHGQMLEEMQSLEEDK NRAIEEAFARAQVEMKAVHE NLAGVRTNLLTLQPALRTL
Function Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance.
Protein Name Kinesin-Like Protein Kifc3
Database Links Reactome: R-HSA-390471 Q9BVG8-2
Cellular Localisation Cell Junction
Adherens Junction
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Apical Cell Membrane
On Membrane Organelles Immediately Beneath The Apical Plasma Membrane Of Renal Tubular Epithelial Cells
Localized In The Distal Tubules And Loops Of Henle In The Kidney
But Not In The Proximal Tubules Or The Glomeruli
With Stronger Staining In The Apical Area Of These Epithelial Cells
Localizes Along Zonula Adherens Only At Mature Cell-Cell Contacts
Alternative Antibody Names Anti-Kinesin-Like Protein Kifc3 antibody
Anti-KIFC3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance