Anti-KIFAP3 antibody (1-200) (STJ24312)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KIFAP3 (1-200) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KIFAP3 |
| Gene ID | 22920 |
| Uniprot ID | KIFA3_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MQGEDARYLKRKVKGGNIDV HPSEKALIVHYEVEATILGE MGDPMLGERKECQKIIRLKS LNANTDITSLARKVVEECKL IHPSKLNEVEQLLYYLQNRR DSLSGKEKKEKSSKPKDPPP FEGMEIDEVANINDMDEYIE LLYEDIPDKVRGSALILQLA RNPDNLEELLLNETALGALA RVLREDWKQSVELATNIIYI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KIFAP3 (NP_055785.2). |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues by SRC in vitro.this reduces the binding affinity of the protein for RAP1GDS1. |
| Function | Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex. |
| Protein Name | Kinesin-Associated Protein 3Kap-3Kap3Smg Gds-Associated Protein |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-2132295Reactome: R-HSA-5620924Reactome: R-HSA-6811434Reactome: R-HSA-983189 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Kinesin-Associated Protein 3 antibodyAnti-Kap-3 antibodyAnti-Kap3 antibodyAnti-Smg Gds-Associated Protein antibodyAnti-KIFAP3 antibodyAnti-KIF3AP antibodyAnti-SMAP antibody |
Information sourced from Uniprot.org