• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g KIFAP3 antibody (STJ24312). Western blot was performed from the immunoprecipitate using KIFAP3 antibody (STJ24312) at a dilution of 1:1000.
  • Immunofluorescence analysis of H9C2 cells using KIFAP3 Rabbit polyclonal antibody (STJ24312) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using KIFAP3 Rabbit polyclonal antibody (STJ24312) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using KIFAP3 Rabbit polyclonal antibody (STJ24312) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using KIFAP3 Rabbit polyclonal antibody (STJ24312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-KIFAP3 antibody (1-200) (STJ24312)

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STJ24312

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIFAP3 (1-200) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIFAP3
Gene ID: 22920
Uniprot ID: KIFA3_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KIFAP3 (NP_055785.2).
Immunogen Sequence: MQGEDARYLKRKVKGGNIDV HPSEKALIVHYEVEATILGE MGDPMLGERKECQKIIRLKS LNANTDITSLARKVVEECKL IHPSKLNEVEQLLYYLQNRR DSLSGKEKKEKSSKPKDPPP FEGMEIDEVANINDMDEYIE LLYEDIPDKVRGSALILQLA RNPDNLEELLLNETALGALA RVLREDWKQSVELATNIIYI
Post Translational Modifications Phosphorylated on tyrosine residues by SRC in vitro.this reduces the binding affinity of the protein for RAP1GDS1.
Function Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex.
Protein Name Kinesin-Associated Protein 3
Kap-3
Kap3
Smg Gds-Associated Protein
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-2132295
Reactome: R-HSA-5620924
Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Alternative Antibody Names Anti-Kinesin-Associated Protein 3 antibody
Anti-Kap-3 antibody
Anti-Kap3 antibody
Anti-Smg Gds-Associated Protein antibody
Anti-KIFAP3 antibody
Anti-KIF3AP antibody
Anti-SMAP antibody

Information sourced from Uniprot.org

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