Anti-KIF4A antibody (870-1080) (STJ112231)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KIF4A (870-1080) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:3000IHC-P:1:50-1:200IF/ICC:1:100-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KIF4A |
| Gene ID | 24137 |
| Uniprot ID | KIF4A_HUMAN |
| Immunogen Region | 870-1080 |
| Immunogen Sequence | LIGELVSSKIQVSKLESSLK QSKTSCADMQKMLFEERNHF AEIETELQAELVRMEQQHQE KVLYLLSQLQQSQMAEKQLE ESVSEKEQQLLSTLKCQDEE LEKMREVCEQNQQLLRENEI IKQKLTLLQVASRQKHLPKD TLLSPDSSFEYVPPKPKPSR VKEKFLEQSMDIEDLKYCSE HSVNEHEDGDGDDDEGDDEE WKPTKLVKVSR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 870-1080 of human KIF4A (NP_036442.3). |
Additional Info
| Tissue Specificity | Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. |
| Function | Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis. Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. |
| Protein Name | Chromosome-Associated Kinesin Kif4aChromokinesin-A |
| Database Links | Reactome: R-HSA-2132295Reactome: R-HSA-437239Reactome: R-HSA-6811434Reactome: R-HSA-983189 |
| Cellular Localisation | Nucleus MatrixCytoplasmCytoskeletonSpindleMidbodyChromosomeAssociates With Chromosomes At All Stage Of MitosisChromatin Localization Is Dependent On Iron-Sulfur Cluster BindingIn AnaphaseAssociates With The Mitotic Spindle MidzoneIn Telophase And CytokinesisCo-Localizes With Ciao2b At The Spindle Midzone And MidbodyCo-Localizes With Prc1 In Early Mitosis And At The Spindle Midzone From Anaphase B To TelophaseDoes Not Localize To The Nucleolus |
| Alternative Antibody Names | Anti-Chromosome-Associated Kinesin Kif4a antibodyAnti-Chromokinesin-A antibodyAnti-KIF4A antibodyAnti-KIF4 antibody |
Information sourced from Uniprot.org