![Anti-Keratin 8+18 antibody [C-51] (STJ16101019)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149735/302476/STJ16101019_1__48411.1646940656.jpg?c=1 "Anti-Keratin 8+18 antibody [C-51] (STJ16101019)")
![Anti-Keratin 8+18 antibody [C-51] (STJ16101019)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149735/302476/STJ16101019_1__48411.1646940656.jpg?c=1 "Anti-Keratin 8+18 antibody [C-51] (STJ16101019)")
Anti-Keratin 8+18 antibody [C-51] (STJ16101019)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion Partner: Sp2/0.
General Information
| Short Description | Mouse monoclonal anti-Keratin 8+18 for use in FC, WB, IF and IHC in Cow, Human, Monkey, Pig and Sheep samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/WB/IF/IHC |
| Host | Mouse |
| Reactivity | Cow/Human/Monkey/Pig/Sheep |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | C-51 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (2-4 µg/ml for 30 |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Immunogen | A BALB/c mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion Partner: Sp2/0. |
Additional Info
| Background | C-51 reacts with keratin 8 (52.5 kDa) + 18 (45 kDa) polypeptides and recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained |
Information sourced from Uniprot.org