Anti-Keratin 5+8 antibody [C-50] (STJ16100253)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mouse was immunized with a cytoskeleton preparation of HeLa cells. Fusion partner: Sp2/0.
STJ16100253
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General Information

Short DescriptionMouse monoclonal anti-Keratin 5+8 for use in FC, WB, IF and IHC in Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat and Sheep samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsFC/WB/IF/IHC
HostMouse
ReactivityCow/Dog/Ferret/Hamster/Human/Mouse/Pig/Rat/Sheep
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDC-50
IsotypeIgG1k
ConjugationUnconjugated
ConcentrationCan be provided as 100 ยตg/mL, 500 ยตg/mL or 1mg/mL.
PurificationAffinity purified from tissue culture.
Dilution RangeFlow Cytometry (1-2 ยตg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4ยฐC, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4ยฐC). Immunoblotting (0.5-1 ยตg/ml). Immunofluorescence (1-2 ยตg/ml). Immunohistology (1-2 ยตg/ml for 30
FormulationPBS with 0.02% Sodium Azide.
Storage InstructionStore for up to 1 year at 2-8ยฐC upon receipt.

Target Information

ImmunogenA BALB/c mouse was immunized with a cytoskeleton preparation of HeLa cells. Fusion partner: Sp2/0.

Additional Info

Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Information sourced from Uniprot.org

Citations

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