![Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149736/302477/STJ16101020_1__58435.1646940657.jpg?c=1 "Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)")
![Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149736/302478/STJ16101020_2__70497.1646940657.jpg?c=1 "Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)")
![Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149736/302477/STJ16101020_1__58435.1646940657.jpg?c=1 "Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)")
Anti-Keratin 13 + 10 antibody [EBS-IF-003] (STJ16101020)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenA hybrid (H2d) mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion partner: Sp2/0.
General Information
| Short Description | Mouse monoclonal anti-Keratin 13 + 10 for use in FC, WB, IF and IHC in Cat, Dog and Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/WB/IF/IHC |
| Host | Mouse |
| Reactivity | Cat/Dog/Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | EBS-IF-003 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (formalin-fixed: 2-4 |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Immunogen | A hybrid (H2d) mouse was immunized with a cytoskeleton preparation from HeLa cells. Fusion partner: Sp2/0. |
Additional Info
| Background | EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis. |
Information sourced from Uniprot.org