• Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using KDM5A antibody (STJ29318) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g KDM5A antibody (STJ29318). Western blot was performed from the immunoprecipitate using KDM5A antibody (STJ29318) at a dilution of 1:500.
  • Western blot analysis of various lysates using KDM5A Rabbit polyclonal antibody (STJ29318) at 1:500 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-KDM5A antibody (STJ29318)

SKU:
STJ29318

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KDM5A is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:100-1:500
ChIP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KDM5A
Gene ID: 5927
Uniprot ID: KDM5A_HUMAN
Immunogen: Recombinant protein of human KDM5A
Immunogen Sequence: Email for sequence
Function Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1. Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells.
Protein Name Lysine-Specific Demethylase 5a
Histone Demethylase Jarid1a
Jumonji/Arid Domain-Containing Protein 1a
Retinoblastoma-Binding Protein 2
Rbbp-2
Histone H3-Trimethyl-L-Lysine(4 Demethylase 5a
Database Links Reactome: R-HSA-3214842
Cellular Localisation Nucleus
Nucleolus
Occupies Promoters Of Genes Involved In Rna Metabolism And Mitochondrial Function
Alternative Antibody Names Anti-Lysine-Specific Demethylase 5a antibody
Anti-Histone Demethylase Jarid1a antibody
Anti-Jumonji/Arid Domain-Containing Protein 1a antibody
Anti-Retinoblastoma-Binding Protein 2 antibody
Anti-Rbbp-2 antibody
Anti-Histone H3-Trimethyl-L-Lysine(4 Demethylase 5a antibody
Anti-KDM5A antibody
Anti-JARID1A antibody
Anti-RBBP2 antibody
Anti-RBP2 antibody

Information sourced from Uniprot.org

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