• Western blot analysis of extracts of various cell lines, using KDM4B/JMJD2B rabbit monoclonal antibody (STJ11102322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of C6 cells using KDM4B/JMJD2B rabbit monoclonal antibody (STJ11102322) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using KDM4B/JMJD2B rabbit monoclonal antibody (STJ11102322) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of HCT116 cells using 3 Mu g KDM4B/JMJD2B antibody (STJ11102322). Western blot was performed from the immunoprecipitate using KDM4B/JMJD2B antibody (STJ11102322) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of 5637; cells, using KDM4B/JMJD2B antibody (STJ11102322) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-KDM4B antibody (50-150) [S2MR] (STJ11102322)

SKU:
STJ11102322

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-KDM4B/JMJD2B (50-150) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KDM4B
Gene ID: 23030
Uniprot ID: KDM4B_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KDM4B/JMJD2B (O94953).
Immunogen Sequence: PPKEWKPRQTYDDIDDVVIP APIQQVVTGQSGLFTQYNIQ KKAMTVGEYRRLANSEKYCT PRHQDFDDLERKYWKNLTFV SPIYGADISGSLYDDDVAQW N
Function Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Plays a critical role in the development of the central nervous system (CNS).
Protein Name Lysine-Specific Demethylase 4b
Jmjc Domain-Containing Histone Demethylation Protein 3b
Jumonji Domain-Containing Protein 2b
Histone H3-Trimethyl-L-Lysine(9 Demethylase 4b
Database Links Reactome: R-HSA-3214842
Reactome: R-HSA-5693565
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Lysine-Specific Demethylase 4b antibody
Anti-Jmjc Domain-Containing Histone Demethylation Protein 3b antibody
Anti-Jumonji Domain-Containing Protein 2b antibody
Anti-Histone H3-Trimethyl-L-Lysine(9 Demethylase 4b antibody
Anti-KDM4B antibody
Anti-JHDM3B antibody
Anti-JMJD2B antibody
Anti-KIAA0876 antibody

Information sourced from Uniprot.org

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