| Host: |
Rabbit |
| Applications: |
WB/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KDM1 (365-515) is suitable for use in Western Blot and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IP 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KDM1A |
| Gene ID: |
23028 |
| Uniprot ID: |
KDM1A_HUMAN |
| Immunogen Region: |
365-515 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 365-515 of human KDM1 (NP_055828.2). |
| Immunogen Sequence: |
ANGQAVPKEKDEMVEQEFNR LLEATSYLSHQLDFNVLNNK PVSLGQALEVVIQLQEKHVK DEQIEHWKKIVKTQEELKEL LNKMVNLKEKIKELHQQYKE ASEVKPPRDITAEFLVKSKH RDLTALCKEYDELAETQGKL EEKLQELEANP |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Polyubiquitinated by JADE2.which leads to its proteasomal degradation. Deubiquitinated by USP38.preventing it from degradation by the 26S proteasome. |
| Function | Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. |
| Protein Name | Lysine-Specific Histone Demethylase 1aBraf35-Hdac Complex Protein Bhc110Flavin-Containing Amine Oxidase Domain-Containing Protein 2Histone H3-Dimethyl-L-Lysine(4 Fad-Dependent Demethylase 1a |
| Database Links | Reactome: R-HSA-3214815Reactome: R-HSA-3214842Reactome: R-HSA-5625886Reactome: R-HSA-8943724Reactome: R-HSA-9018519Reactome: R-HSA-9029569Reactome: R-HSA-9679191Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Lysine-Specific Histone Demethylase 1a antibodyAnti-Braf35-Hdac Complex Protein Bhc110 antibodyAnti-Flavin-Containing Amine Oxidase Domain-Containing Protein 2 antibodyAnti-Histone H3-Dimethyl-L-Lysine(4 Fad-Dependent Demethylase 1a antibodyAnti-KDM1A antibodyAnti-AOF2 antibodyAnti-KDM1 antibodyAnti-KIAA0601 antibodyAnti-LSD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
