• STJ71861 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-KCNQ1 antibody (C-Term) (STJ71861)

SKU:
STJ71861-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-KCNQ1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: KCNQ1
Gene ID: 3784
Uniprot ID: KCNQ1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000209.2; NP_861463.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_000209.2; NP_861463.1).
Immunogen Sequence: EQLTVPRRGPDEGS
Post Translational Modifications Phosphorylation at Ser-27 by PKA.increases delayed rectifier potassium channel activity of the KCNQ1-KCNE1 complex through a macromolecular complex that includes PKA, PP1, and the targeting protein AKAP9. Ubiquitinated by NEDD4L.promotes internalization. The ubiquitinylated form is internalized through a clathrin-mediated endocytosis by interacting with AP2M1 and is recycled back to the cell membrane via RAB4A and RAB11A. Deubiquitinated by USP2.counteracts the NEDD4L-specific down-regulation of I(Ks) and restores the membrane localization.
Function Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon. Associates with KCNE beta subunits that modulates current kinetics. Induces a voltage-dependent current by rapidly activating and slowly deactivating potassium-selective outward current. Promotes also a delayed voltage activated potassium current showing outward rectification characteristic. During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current. When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions. This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents. During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion. Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion. When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current. When associated with KCNE4, inhibits voltage-gated potassium channel activity. When associated with KCNE5, this complex only conducts current upon strong and continued depolarization. Also forms a heterotetramer with KCNQ5.has a voltage-gated potassium channel activity. Binds with phosphatidylinositol 4,5-bisphosphate. KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability. Isoform 2: Non-functional alone but modulatory when coexpressed with the full-length isoform 1.
Protein Name Potassium Voltage-Gated Channel Subfamily Kqt Member 1
Iks Producing Slow Voltage-Gated Potassium Channel Subunit Alpha Kvlqt1
Kqt-Like 1
Voltage-Gated Potassium Channel Subunit Kv7.1
Database Links Reactome: R-HSA-1296072
Reactome: R-HSA-5576890
Reactome: R-HSA-5576893
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle Membrane
Early Endosome
Membrane Raft
Endoplasmic Reticulum
Basolateral Cell Membrane
Apical Cell Membrane
Colocalized With Kcne3 At The Plasma Membrane
Upon 17beta-Oestradiol Treatment
Colocalizes With Rab5a At Early Endosome
Heterotetramer With Kcnq5 Is Highly Retained At The Endoplasmic Reticulum And Is Localized Outside Of Lipid Raft Microdomains
During The Early Stages Of Epithelial Cell Polarization Induced By The Calcium Switch
It Is Removed From The Plasma Membrane To The Endoplasmic Reticulum
Where It Is Retained
And Redistributed To The Basolateral Cell Surface In A Pi3k-Dependent Manner At A Later Stage
Colocalizes With Slc5a3 At The Apical Membrane Of Choroid Plexus Epithelium
Alternative Antibody Names Anti-Potassium Voltage-Gated Channel Subfamily Kqt Member 1 antibody
Anti-Iks Producing Slow Voltage-Gated Potassium Channel Subunit Alpha Kvlqt1 antibody
Anti-Kqt-Like 1 antibody
Anti-Voltage-Gated Potassium Channel Subunit Kv7.1 antibody
Anti-KCNQ1 antibody
Anti-KCNA8 antibody
Anti-KCNA9 antibody
Anti-KVLQT1 antibody

Information sourced from Uniprot.org

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