Anti-KCNJ1/ROMK antibody (Internal) (STJ71817)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-KCNJ1/ROMK (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 44.8kDa according to NP_000211.1). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | KCNJ1 |
| Gene ID | 3758 |
| Uniprot ID | KCNJ1_HUMAN |
| Accession Number | NP_000211.1; NP_722448.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | DQININFVVDAGNEN |
| Specificity | This antibody is expected to recognize reported isoforms NP_722449.2 and NP_000211. The following reported variants represent identical protein: NP_722451.1, NP_722449.2, NP_722450.1, NP_722448.1. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane. |
| Function | Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium.as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium. In the kidney, probably plays a major role in potassium homeostasis. |
| Protein Name | Atp-Sensitive Inward Rectifier Potassium Channel 1Atp-Regulated Potassium Channel Rom-KInward Rectifier K(+ Channel Kir1.1Potassium Channel - Inwardly Rectifying Subfamily J Member 1 |
| Database Links | Reactome: R-HSA-1296067 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPhosphorylation At Ser-44 By Sgk1 Is Necessary For Its Expression At The Cell Membrane |
| Alternative Antibody Names | Anti-Atp-Sensitive Inward Rectifier Potassium Channel 1 antibodyAnti-Atp-Regulated Potassium Channel Rom-K antibodyAnti-Inward Rectifier K(+ Channel Kir1.1 antibodyAnti-Potassium Channel - Inwardly Rectifying Subfamily J Member 1 antibodyAnti-KCNJ1 antibodyAnti-ROMK1 antibody |
Information sourced from Uniprot.org