| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-KCC3/SLC12A6 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-0.1-0.3µg/mlIHC-Recommended concentration, 2-4µg/mlELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
SLC12A6 |
| Gene ID: |
9990 |
| Uniprot ID: |
S12A6_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; NP_005126.1; NP_598408.1 |
| Specificity: |
This antibody is expected to recognise all reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1) ; Reported variants represent identicial protein: NP_001035959.1; NP_001035960.1 |
| Immunogen Sequence: |
SRHIDVCSKTKE |
| Post Translational Modifications | Phosphorylated, phosphorylation regulates transporter activity. Phosphorylated at Thr-991 and Thr-1048 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. N-glycosylated. |
| Function | Isoform 1: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. Isoform 2: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 3: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 4: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 5: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 6: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). |
| Protein Name | Solute Carrier Family 12 Member 6Electroneutral Potassium-Chloride Cotransporter 3K-Cl Cotransporter 3 |
| Database Links | Reactome: R-HSA-426117Reactome: R-HSA-5619039 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell Membrane |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 6 antibodyAnti-Electroneutral Potassium-Chloride Cotransporter 3 antibodyAnti-K-Cl Cotransporter 3 antibodyAnti-SLC12A6 antibodyAnti-KCC3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
