• Western blot analysis of lysates from Rat lung, using KBTBD8 Rabbit polyclonal antibody (STJ11100464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-KBTBD8 antibody (50-280) (STJ11100464)

SKU:
STJ11100464

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KBTBD8 (50-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KBTBD8
Gene ID: 84541
Uniprot ID: KBTB8_HUMAN
Immunogen Region: 50-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-280 of human KBTBD8 (NP_115894.2).
Immunogen Sequence: DIVVEVDHGKTFSCHRNVLA AISPYFRSMFTSGLTESTQK EVRIVGVEAESMDLVLNYAY TSRVILTEANVQALFTAASI FQIPSIQDQCAKYMISHLDP QNSIGVFIFADHYGHQELGD RSKEYIRKKFLCVTKEQEFL QLTKDQLISILDSDDLNVDR EEHVYESIIRWFEHEQNERE VHLPEIFAKCIRFPLMEDTF IEKIPPQFAQAIAKSCVEKG PSNTNGCTQRL
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification. The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.
Protein Name Kelch Repeat And Btb Domain-Containing Protein 8
T-Cell Activation Kelch Repeat Protein
Ta-Krp
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Golgi Apparatus
Translocates To The Spindle Apparatus During Mitosis
Alternative Antibody Names Anti-Kelch Repeat And Btb Domain-Containing Protein 8 antibody
Anti-T-Cell Activation Kelch Repeat Protein antibody
Anti-Ta-Krp antibody
Anti-KBTBD8 antibody
Anti-KIAA1842 antibody
Anti-TAKRP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance