• Confocal imaging of paraffin-embedded Rat kidney using KAT8/MYST1/MOF Rabbit monoclonal antibody (STJ11101860, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of U-2 OS cells using KAT8/MYST1/MOF Rabbit monoclonal antibody (STJ11101860, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of KAT8/MYST1/MOF in paraffin-embedded human appendix using KAT8/MYST1/MOF Rabbit monoclonal antibody (STJ11101860) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using KAT8/MYST1/MOF Rabbit monoclonal antibody (STJ11101860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-KAT8 antibody (350-458) [S0MR] (STJ11101860)

SKU:
STJ11101860

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-KAT8/MYST1/MOF (350-458) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KAT8
Gene ID: 84148
Uniprot ID: KAT8_HUMAN
Immunogen Region: 350-458
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-458 of human KAT8/MYST1/MOF (Q9H7Z6).
Immunogen Sequence: EKPLSDLGKLSYRSYWSWVL LEILRDFRGTLSIKDLSQMT SITQNDIISTLQSLNMVKYW KGQHVICVTPKLVEEHLKSA QYKKPPITVDSVCLKWAPPK HKQVKLSKK
Post Translational Modifications Autoacetylation at Lys-274 is required for binding histone H4 with high affinity and for proper function.
Function Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.
Protein Name Histone Acetyltransferase Kat8
Lysine Acetyltransferase 8
Moz - Ybf2/Sas3 - Sas2 And Tip60 Protein 1
Myst-1
Hmof
Database Links Reactome: R-HSA-3214847
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone Acetyltransferase Kat8 antibody
Anti-Lysine Acetyltransferase 8 antibody
Anti-Moz - Ybf2/Sas3 - Sas2 And Tip60 Protein 1 antibody
Anti-Myst-1 antibody
Anti-Hmof antibody
Anti-KAT8 antibody
Anti-MOF antibody
Anti-MYST1 antibody
Anti-PP7073 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance